CALL FOR PAPERS Comparative Genomics Expression of genes involved in excitatory neurotransmission in anoxic crucian carp (Carassius carassius) brain
نویسندگان
چکیده
Stian Ellefsen, Guro K. Sandvik, Helene K. Larsen, Kåre-Olav Stensløkken, Dag Are S. Hov, Tom A. Kristensen, and Göran E. Nilsson Physiology Programme, Department of Molecular Biosciences, University of Oslo, Oslo; Lillehammer University College, Lillehammer; Cancer and Surgical Division, Ullevål University Hospital, Oslo; and Gene Programme, Department of Molecular Biosciences, University of Oslo, Oslo, Norway
منابع مشابه
Expression of genes involved in excitatory neurotransmission in anoxic crucian carp (Carassius carassius) brain.
The crucian carp, Carassius carassius, survives months without oxygen. During anoxia it needs to keep energy expenditure low, particularly in the brain, with its high rate of ATP use related to neuronal activity. This could be accomplished by reducing neuronal excitability through altered expression of genes involved in excitatory neurotransmission. Through cloning and the use of a recently dev...
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The crucian carp, Carassius carassius, survives days to months without oxygen, depending on temperature. In the anoxic crucian carp brain, increased GABAergic inhibition, mediated by increased extracellular levels of GABA, has been shown to suppress electric activity and ATP consumption. To investigate an involvement of gene expression in this response, we utilized real-time RT-PCR to test the ...
متن کاملExpression of heat shock proteins in anoxic crucian carp (Carassius carassius): support for cold as a preparatory cue for anoxia.
The crucian carp (Carassius carassius) tolerates anoxia for days to months depending on temperature. During episodes of stress, heat shock proteins (HSPs) are important for limiting cellular damage, mainly by ensuring protein function. Accordingly, we hypothesized that anoxia would change the expression of HSPs and that this response would be temperature dependent. Real-time RT-PCR was used to ...
متن کاملIntrinsic contractile properties of the crucian carp (Carassius carassius) heart during anoxic and acidotic stress.
The crucian carp (Carassius carassius) seems unique among vertebrates in its ability to maintain cardiac performance during prolonged anoxia. We investigated whether this phenomenon arises in part from a myocardium tolerant to severe acidosis or because the anoxic crucian carp heart may not experience a severe extracellular acidosis due to the fish's ability to convert lactate to ethanol. Spont...
متن کاملDifferential regulation of AMP-activated kinase and AKT kinase in response to oxygen availability in crucian carp (Carassius carassius).
We investigated whether two kinases critical for survival during periods of energy deficiency in anoxia-intolerant mammalian species, AMP-activated kinase (AMPK), and protein kinase B (AKT), are equally important for hypoxic/anoxic survival in the extremely anoxia-tolerant crucian carp (Carassius carassius). We report that phosphorylation of AMPK and AKT in heart and brain showed small changes ...
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